Colorado State University-Pueblo 5 articles published in JoVE Biology Mouse Lumbar Vertebra Uniaxial Compression Testing with Embedding of the Loading Surface Chandler A. Sparks1,2, Ryeim B. Ansaf1,3, Annette M. Gabaldón1 1Department of Biology, Colorado State University-Pueblo, 2Hackensack Meridian School of Medicine, 3Department of Engineering, Colorado State University-Pueblo In this protocol, two approaches are described to make uniaxial compression testing of mouse lumbar vertebrae more attainable. First, the conversion of a three-point bending machine to a compression testing machine is described. Second, an embedding method for preparing the loading surface that uses bone cement is adapted for mouse lumbar vertebrae. Biochemistry Ultrasonic-Assisted Extraction of Cannabidiolic Acid from Cannabis Biomass Kenneth J. Olejar*1, Min Hong*2, Sun-Yeop Lee2, Tae-Hyung Kwon2, Soo-Ung Lee2, Chad A. Kinney1,3, Joon-Hee Han2, Sang-Hyuck Park3 1Department of Chemistry, Colorado State University-Pueblo, 2Chuncheon Bioindustry Foundation, 3Institute for Cannabis Research, Colorado State University-Pueblo Ultrasonic-assisted extraction (UAE) increases extraction efficiency of solvents and when applied to Cannabis spp. biomass it reduces the time required for extraction. This decreases the cost and potential cannabinoid loss due to degradation. Additionally, UAE is considered a green method due to low solvent use. Biology Tobacco Hornworm as an Insect Model System for Cannabinoid Pre-clinical Studies Sang-Hyuck Park1, Samuel Koch2, Katherine Richardson3, Christopher Pauli1, Joon-Hee Han4, Tae-Hyung Kwon4 1Institute of Cannabis Research, Colorado State University-Pueblo, 2Department of Biology, Colorado State University-Pueblo, 3Department of Chemistry, Colorado State University-Pueblo, 4Chuncheon Bioindustry Foundation The present protocol provides instructional information for using tobacco hornworm Manduca sexta in cannabinoid research. The method described here includes all necessary supplies and protocols to monitor physiological and behavioral changes of the insect model in response to cannabidiol (CBD) treatment. Bioengineering Employing Aeroponic Systems for the Clonal Propagation of Cannabis Trevor Regas1, Joon-Hee Han2, Christopher S. Pauli3, Sang-Hyuck Park1,3 1Biology Department, Colorado State University-Pueblo, 2Chuncheon Bioindustry Foundation, 3Institute of Cannabis Research, Colorado State University-Pueblo This protocol is designed to provide instructional information for the clonal propagation of Cannabis sativa L. by implementing aeroponic systems. The method described here includes all necessary supplies and protocols to successfully reproduce desirable morphological and chemical properties in the genus Cannabis. Biology Experimental Protocol for Using Drosophila As an Invertebrate Model System for Toxicity Testing in the Laboratory Elizabeth K. Peterson1,2, Hugh E. Long1,2 1Communities for Building Active STEM Engagement, Colorado State University-Pueblo, 2Department of Biology, Colorado State University-Pueblo In this paper, we provide a detailed protocol for exposing species in the genus Drosophila to pollutants with the goal of studying the impact of exposure on a range of phenotypic outputs at different developmental stages and for more than one generation.
