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1 article published in JoVE
Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence Dana Faratian1, Jason Christiansen2, Mark Gustavson2, Christine Jones2, Christopher Scott2, InHwa Um1, David J. Harrison1 1Division of Pathology, University of Edinburgh, 2HistoRx Inc. Here we describe a method to quantify molecular heterogeneity in histological sections of tumor material using quantitative immunofluorescence, image analysis, and a statistical measure of heterogeneity. The method is intended for use in clinical biomarker development and analysis.