3 articles published in JoVE
Methods for Measuring the Orientation and Rotation Rate of 3D-printed Particles in Turbulence Brendan C. Cole1, Guy G. Marcus1, Shima Parsa1, Stefan Kramel1, Rui Ni1, Greg A. Voth1 1Department of Physics, Wesleyan University We use 3D printing to fabricate anisotropic particles in the shapes of jacks, crosses, tetrads, and triads, whose alignments and rotations in turbulent fluid flow can be measured from multiple simultaneous video images.
Live-imaging of the Drosophila Pupal Eye Mark B. Hellerman1, Richard H. Choe1, Ruth I. Johnson1 1Biology Department, Wesleyan University This protocol presents an efficient method for imaging the live Drosophila pupal eye neuroepithelium. This method compensates for tissue movement and uneven topology, enhances visualization of cell boundaries through the use of multiple GFP-tagged junction proteins, and uses an easily-assembled imaging rig.
Application of Stopped-flow Kinetics Methods to Investigate the Mechanism of Action of a DNA Repair Protein F. Noah Biro1, Jie Zhai1, Christopher W. Doucette1, Manju M. Hingorani1 1Molecular Biology and Biochemistry Department, Wesleyan University Msh2-Msh6 is responsible for initiating repair of replication errors in DNA. Here we present a transient kinetics approach towards understanding how this critical protein works. The report illustrates stopped-flow experiments for measuring the coupled DNA binding and ATPase kinetics underlying Msh2-Msh6 mechanism of action in DNA repair.