Lombardi Comprehensive Cancer Center 1 article published in JoVE Cancer Research A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation Lucas Tricoli1, Deborah L. Berry2, Chris Albanese1 1Department of Oncology, Lombardi Comprehensive Cancer Center, 2Department of Oncology, Georgetown University Medical Center Here, we present a method for the establishment of a rapid in vitro system that supports the three dimensional culturing and subsequent luminal differentiation of primary prostate epithelial cells.