Georgetown University, School of Nursing and Health Studies 1 article published in JoVE Cancer Research In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis Sung-Hyeok Hong*1, Jason U. Tilan*2,3, Susana Galli1, Rachel Acree3, Katherine Connors4, Akanksha Mahajan1, Larissa Wietlisbach3, Taylor Polk3, Ewa Izycka-Swieszewska5, Yi-Chien Lee6, Luciane R. Cavalli6, Olga C. Rodriguez6, Chris Albanese6,7, Joanna B. Kitlinska1 1Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, 2Department of Nursing, Georgetown University, School of Nursing and Health Studies, 3Department of Human Science, Georgetown University, School of Nursing and Health Studies, 4School of Medicine, Georgetown University Medical Center, 5Department of Pathology and Neuropathology, Medical University of Gdańsk, 6Department of Oncology, Georgetown University Medical Center, 7Department of Pathology, Georgetown University Medical Center This manuscript describes the development of an animal model that allows for the direct testing of the effects of tumor hypoxia on metastasis and the deciphering the mechanisms of its action. Although the experiments described here focus on Ewing sarcoma, a similar approach can be applied to other tumor types.
