2 articles published in JoVE
Measuring Carbon-based Contaminant Mineralization Using Combined CO2 Flux and Radiocarbon Analyses Thomas J. Boyd1, Michael T. Montgomery1, Richard H. Cuenca2, Yutaka Hagimoto2 1Marine Biogeochemistry, Code 6114, US Naval Research Laboratory, 2Department of Biological and Ecological Engineering, Oregon State University A protocol is described wherein CO2 mineralized from organic contaminant (derived from petroleum feedstocks) biodegradation is trapped, quantified, and analyzed for 14C content. A model is developed to determine CO2 capture zone's spatial extent. Spatial and temporal measurements allow integrating contaminant mineralization rates for predicting remediation extent and time.
Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape Darryl A. Boyd1, Andre A. Adams1, Michael A. Daniele1, Frances S. Ligler1,2 1Center for Bio/Molecular Science & Engineering, US Naval Research Laboratory, 2Joint Department of Biomedical Engineering, North Carolina State University and University of North Carolina at Chapel Hill Two adjacent fluids passing through a grooved microfluidic channel can be directed to form a sheath around a prepolymer core; thereby determining both shape and cross-section. Photoinitiated polymerization, such as thiol click chemistry, is well suited for rapidly solidifying the core fluid into a microfiber with predetermined size and shape.