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4 articles published in JoVE
Creating Objects and Object Categories for Studying Perception and Perceptual Learning Karin Hauffen1,2,3, Eugene Bart4, Mark Brady5, Daniel Kersten6, Jay Hegdé1,2,3 1Brain and Behavior Discovery Institute, Georgia Health Sciences University, 2Vision Discovery Institute, Georgia Health Sciences University, 3Department of Opthalmology, Georgia Health Sciences University, 4Intelligent Systems Laboratory, Palo Alto Research Center, 5Pattern Recognition Systems, Palo Alto Research Center, 6Department of Psychology, University of Minnesota We describe a novel methodology for creating naturalistic 3-D objects and object categories with precisely defined feature variations. We use simulations of the biological processes of morphogenesis and phylogenesis to create novel, naturalistic virtual 3-D objects and object categories that can then be rendered as visual images or haptic objects.
Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction Erhard Bieberich1 1Institute of Molecular Medicine and Genetics, Georgia Health Sciences University To test the interaction of a protein with its target lipid we used MACS and Annexin V-conjugated magnetic beads and lipid vesicles synthesized from the target lipid and Annexin V-binding phosphatidylserine. Proteins bound to the target lipid are co-purified and analyzed after elution from the beads.
Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model Mary Zimmerman*1, Xiaolin Hu*1, Kebin Liu1 1Department of Biochemistry and Molecular Biology, Medical College of Georgia An experimental lung metastasis and CTL immunotherapy mouse model for analysis of tumor cells-T cell interaction in vivo.
Multicolor Time-lapse Imaging of Transgenic Zebrafish: Visualizing Retinal Stem Cells Activated by Targeted Neuronal Cell Ablation Junko Ariga*1, Steven L. Walker*1, Jeff S. Mumm1 1Department of Cellular Biology and Anatomy, Medical College of Georgia In this video, techniques for multicolor confocal time-lapse imaging and targeted cell ablation are provided. Time-lapse imaging is used to monitor the behavior of multiple cell types of interest in vivo. Targeted cell ablation facilitates the study neural circuit function and cell-specific neuronal regeneration paradigms.