3 articles published in JoVE
Systems Analysis of the Neuroinflammatory and Hemodynamic Response to Traumatic Brain Injury Rowan O. Brothers*1, Sara Bitarafan*2,3, Alyssa F. Pybus1,3, Levi B. Wood*1,2,3, Erin M. Buckley*1,4,5 1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 2George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 3Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 4Department of Pediatrics, Emory University School of Medicine, 5 This protocol presents methods to characterize the neuroinflammatory and hemodynamic response to mild traumatic brain injury and to integrate these data as part of a multivariate systems analysis using partial least squares regression.
A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry M. Edward Quach1,2, Anum K. Syed1,2, Renhao Li1,2 1 We describe an in-solution method to apply uniform shear to platelet surface receptors using cone-plate viscometry. This method may also be used more broadly to apply shear to other cell types and cell-fragments and need not target a specific ligand-receptor pair.
Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases David R. Myers*1,2,3,4, Yumiko Sakurai*1,2,3,4, Reginald Tran1,2,3,4, Byungwook Ahn1,2,3,4, Elaissa Trybus Hardy1,2,3,4, Robert Mannino1,2,3,4, Ashley Kita1,2,3,4, Michelle Tsai1,2,3,4, Wilbur A. Lam1,2,3,4 1Department of Pediatrics, Emory University School of Medicine, 2Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 3Aflac Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta, 4Winship Cancer Institute of Emory University A method to culture an endothelial cell monolayer throughout the entire inner 3D surface of a microfluidic device with microvascular-sized channels (<30 μm) is described. This in vitro microvasculature model enables the study of biophysical interactions between blood cells, endothelial cells, and soluble factors in hematologic diseases.