3 articles published in JoVE
Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands Stavros Maurakis1, Cynthia Nau Cornelissen1 1Institute for Biomedical Sciences, Georgia State University We describe here a method for growth of Neisseria gonorrhoeae in metal-restricted liquid medium to facilitate the expression of genes for metal uptake. We also outline downstream experiments to characterize the phenotype of gonococci grown in these conditions. These methods may be adapted to be suitable for characterization of metal-responsive genes in other bacteria.
A High-content Assay for Monitoring AMPA Receptor Trafficking Mohammad A. Ghane*1, Dina W. Yakout*1, Angela M. Mabb1 1Neuroscience Institute, Georgia State University Neuronal excitability can be modulated through a dynamic process of endo- and exocytosis of excitatory ionotropic glutamate receptors. Described here is an accessible, high-content assay for quantifying surface and internal receptor population pools.
Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+ Florence N. Reddish1, Cassandra L. Miller1, Rakshya Gorkhali1, Jenny J. Yang1 1Department of Chemistry, Center of Diagnostics and Therapeutics (CDT), Georgia State University We present protocols for the application of our targeted genetically-encoded calcium indicator (GECI) CatchER+ for monitoring rapid calcium transients in the endoplasmic/sarcoplasmic reticulum (ER/SR) of HEK293 and skeletal muscle C2C12 cells using real-time fluorescence microscopy. A protocol for the in situ Kd measurement and calibration is also discussed.