3 articles published in JoVE
A Rat Model of Ventricular Fibrillation and Resuscitation by Conventional Closed-chest Technique Lorissa Lamoureux1, Jeejabai Radhakrishnan1, Raúl J. Gazmuri1 1Resuscitation Institute, Rosalind Franklin University of Medicine and Science This article describes a rat model of electrically-induced ventricular fibrillation and resuscitation by chest compression, ventilation, and delivery of electrical shocks that simulates an episode of sudden cardiac arrest and conventional cardiopulmonary resuscitation. The model enables gathering insights on the pathophysiology of cardiac arrest and exploration of new resuscitation strategies.
Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets Stephanie Flaherty1, Joseph M. Reynolds1 1Department of Microbiology and Immunology, The Chicago Medical School, Rosalind Franklin University of Medicine and Science Naïve CD4+ T cells polarize to various subsets depending on the environment at the time of activation. The differentiation of naïve CD4+ T cells to various effector subsets can be achieved in vitro through the addition of T cell receptor stimuli and specific cytokine signals.
A Zebrafish Model of Diabetes Mellitus and Metabolic Memory Robert V. Intine1, Ansgar S. Olsen1, Michael P. Sarras Jr.2 1Dr. William M. Scholl College of Podiatric Medicine, Rosalind Franklin University of Medicine and Science, 2Chicago Medical School, Rosalind Franklin University of Medicine and Science Metabolic memory is the phenomenon by which diabetic complications persist and progress unimpeded even after euglycemia is achieved pharmaceutically. Here we describe a diabetes mellitus zebrafish model which is unique in that it allows for the examination of the mitotically transmissible epigenetic components of metabolic memory in vivo.