2 articles published in JoVE
Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) Procedure Erik Schadde1,2,3, Martin Hertl2, Stefan Breitenstein3, Beatrice Beck-Schimmer1,4, Martin Schläpfer1,4 1Institute of Physiology - Center for Integrative Human Physiology, University of Zurich, 2Department of Surgery, Rush University Medical Center, 3Department of Surgery, Cantonal Hospital Winterthur, 4Institute of Anesthesiology, University and University Hospital Zurich Inducing rapid liver hypertrophy using Associating Liver Partition and Portal vein ligation for a Staged hepatectomy (ALPPS) has been proposed for resection of borderline resectable liver tumors. This model may elucidate mechanisms involved in rapid hypertrophy and allows testing of drugs that promote or block the acceleration of regeneration.
Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers Ki Ho Park1, Noah Weisleder2, Jingsong Zhou3, Kristyn Gumpper1, Xinyu Zhou1, Pu Duann4, Jianjie Ma1, Pei-Hui Lin1 1Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, 2Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, 3Department of Molecular Biophysics and Physiology, Rush University Medical Center, 4Department of Internal Medicine, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center Described here is a method to directly measure calcium sparks, the elementary units of Ca2+ release from sarcoplasmic reticulum in intact skeletal muscle fibers. This method utilizes osmotic-stress-mediated triggering of Ca2+ release from ryanodine receptor in isolated muscle fibers. The dynamics and homeostatic capacity of intracellular Ca2+ signaling can be employed to assess muscle function in health and disease.