Stoelting Co. Wood Dale 1 article published in JoVE Behavior Assessment of Nerve Injury-Induced Mechanical Hypersensitivity in Rats Using an Orofacial Operant Pain Assay Basak Donertas-Ayaz1, Ariana C. Brice-Tutt2, Wendi L. Malphurs2, Darrice Montgomery2, Richard H. Mills3, John K. Neubert2, Robert M. Caudle1 1Department of Oral and Maxillofacial Surgery, University of Florida, Gainesville, 2Department of Orthodontics, University of Florida, Gainesville, 3Stoelting Co. Wood Dale This protocol describes the assessment of mechanical hypersensitivity in a rat model of neuropathic orofacial pain using an operant-based orofacial pain assessment device.