2 articles published in JoVE
Cell Population Analyses During Skin Carcinogenesis Dongsheng Gu1, Qipeng Fan1, Jingwu Xie1 1Department of Pediatrics, Wells Center for Pediatric Research, IU Simon Cancer Center, Indiana University Carcinogenesis is a process involving interactions between cancer cells and the cancer microenvironment. To dissect the molecular events, one needs to isolate different cell populations at different stages during cancer development. Using a mouse model for basal cell carcinoma, we describe a protocol for cell analyses during carcinogenesis.
Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels Asad Raza1, Chien-Chi Lin1 1Department of Biomedical Engineering, Purdue School of Engineering and Technology, Indiana University - Purdue University at Indianapolis The following protocol provides techniques for encapsulating pancreatic β-cells in step-growth PEG-peptide hydrogels formed by thiol-ene photo-click reactions. This material platform not only offers a cytocompatible microenvironment for cell encapsulation, but also permits user-controlled rapid recovery of cell structures formed within the hydrogels.