Butler University 1 article published in JoVE Genetics Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer Chioniso Patience Masamha1, Zachery Todd1 1Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Butler University The two different 3' rapid amplification of cDNA ends (3' RACE) protocols described here make use of two different DNA polymerases to map sequences that include a segment of the open reading frame (ORF), the stop codon, and the entire 3' UTR of a transcript using RNA obtained from different cancer cell lines.
