3 articles published in JoVE
Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry Haley R. Pugsley1 1Amnis Biology Department, MilliporeSigma Here, multispectral imaging flow cytometry with an analytical feature that compares bright detail images of 3 autophagy markers and quantifies their co-localization, along with LC3 spot counting, was used to measure autophagy in an objective, quantitative, and statistically robust manner.
Determining Cell Number During Cell Culture using the Scepter Cell Counter Kathleen Ongena1, Chandreyee Das1, Janet L. Smith1, Sónia Gil1, Grace Johnston1 1Bioscience Division, Millipore Inc The Scepter Cell Counter is a handheld automated device that can be used to count cells, monitor cell diameter and volume, and be used to check the health and quality of cellular populations from one culture to the next.
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity Janet L Anderl1, Stella Redpath1, Andrew J Ball1 1Bioscience Division, High Content Analysis Research and Development, Millipore Inc This article describes a novel protocol and reagent set designed for sensitive measurement of neurotoxic effects of compounds and treatments on co-cultures of neurons and astrocytes using high content analysis. Results demonstrate that high content analysis represents an exciting novel technology for neurotoxicity assessment.