3 articles published in JoVE
Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer Krissy Remple1, Laurel Stone2 1Department of Molecular and Cell Biology, Life Technologies, 2Life Technologies This protocol describes how to perform cell viability and fluorescence expression assays using the Tali Image-Based Cytometer.
Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium Kate Wagner1, David Welch1 1GIBCO, Life Technologies The following protocol provides instruction for adapting human induced Pluripotent Stem (iPS) Cells to feeder-free culture using complete KnockOut Serum Replacement Feeder-Free medium (KSR-FF). Once adapted, instructions for continual maintenance are also provided.
Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium Kate Wagner1, David Welch1 1GIBCO, Life Technologies This protocol describes the detailed procedure for cryopreserving human iPS cells in KnockOut SR cryopreservation medium and recovering these cells in complete KnockOut SR Feeder Free (KSR-FF) medium or feeder-based KnockOut SR medium.