6 articles published in JoVE

• Neuroscience

  
  Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants Mauro W. Zappaterra¹, Anthony S. LaMantia², Christopher A. Walsh^3,4, Maria K. Lehtinen^{5 1}Department of Physical Medicine and Rehabilitation, VA Greater Los Angeles Healthcare System, ²Department of Pharmacology and Physiology, Institute for Neuroscience, The George Washington University School of Medicine and Health Sciences, ³Division of Genetics, Department of Medicine, Boston Children's Hospital, ⁴Howard Hughes Medical Institute, Boston Children's Hospital, ⁵Department of Pathology, Boston Children's Hospital, Harvard Medical School The ventricular cerebrospinal fluid (CSF) bathes the neuroepithelial and cerebral cortical progenitor cells during early brain development in the embryo. Here we describe the method developed to isolate ventricular CSF from rodent embryos of different ages in order to investigate its biological function. In addition, we demonstrate our cerebral cortical explant dissection and culture technique that allows for explant growth with minimal volumes of culture medium or CSF.

• Immunology and Infection

  
  Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade Jeff K. Davies^1,2, Christine M. Barbon¹, Annie R. Voskertchian¹, Lee M. Nadler^1,2, Eva C. Guinan^{3,4 1}Medical Oncology, Dana Farber Cancer Institute, ²Department of Medicine, Brigham and Womens Hospital, ³Pediatric Oncology, Dana Farber Cancer Institute, ⁴Division of Hematology/Oncology, Children’s Hospital Boston This paper describes a simple technique to induce alloantigen-specific anergy in human peripheral blood mononuclear cells. The technique can be applied clinically to generate non-alloreactive donor cells. Infusion of these cells could improve immune reconstitution and reduce toxicity after allogeneic hematopoietic stem cell transplantation.

• Biology

  
  Retro-orbital Injection in Adult Zebrafish Emily K. Pugach^1,2, Pulin Li^1,2, Richard White^1,2,3, Leonard Zon^{1,2 1}Department of Hematology and Oncology, Children’s Hospital Boston, ²Harvard Stem Cell Institute, Harvard Medical School, ³Department of Medical Oncology, Dana Farber Cancer Institute Here we show how to do retro-orbital injection in adult zebrafish.

• Biology

  
  Making MR Imaging Child's Play - Pediatric Neuroimaging Protocol, Guidelines and Procedure Nora M. Raschle^1,2, Michelle Lee¹, Roman Buechler¹, Joanna A. Christodoulou³, Maria Chang¹, Monica Vakil¹, Patrice L. Stering¹, Nadine Gaab^{1,3,4 1}Department of Developmental Medicine, Children’s Hospital Boston, ²Department of Neuropsychology, University of Zurich, ³Graduate School of Education, Harvard, ⁴Harvard Medical School Despite an increase in the use of structural and functional magnetic resonance imaging (fMRI) in humans, the study of young pediatric populations remains a challenge. We present a hands-on, step-by-step video protocol including guidelines for clinicians and researchers intending to perform (f)MRI in young children.

• Biology

  
  Implantation of Engineered Tissue in the Rat Heart Bjoern Sill¹, Ivan V. Alpatov², Christina A. Pacak², Douglas B. Cowan^{2 1}Department of Anesthesiology, Perioperative and Pain Medicine, Children's Hospital Boston and Harvard Medical School, ²Department of Anesthesiology, Perioperative and Pain Medicine, Children’s Hospital Boston Here, we describe a cardiac surgical procedure to implant engineered tissue in the atrioventricular (AV)-groove of an adult Lewis rat.

• Biology

  
  Microinjection of Zebrafish Embryos to Analyze Gene Function Jonathan N. Rosen^1,2, Michael F. Sweeney¹, John D. Mably^{1,2 1}Department of Genetics, Harvard Medical School, ²Department of Cardiology, Children’s Hospital Boston This video shows how morpholino or mRNA can be injected into zebrafish embryos at the one-cell stage to decrease or increase the level of specific gene products during subsequent development.