2 articles published in JoVE
Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization Dania M. Figueroa1, Heidi M. Wade1,2, Katrina P. Montales2, Donald E. Elmore1,2, Louise E.O. Darling1,3 1Biochemistry Program, Wellesley College, 2Department of Chemistry, Wellesley College, 3Department of Biological Sciences, Wellesley College Here we present a protocol to produce gram-negative Escherichia coli (E. coli) spheroplasts and gram-positive Bacillus megaterium (B. megaterium) protoplasts to clearly visualize and rapidly characterize peptide-bacteria interactions. This provides a systematic method to define membrane localizing and translocating peptides.
Measuring Peptide Translocation into Large Unilamellar Vesicles Sara A. Spinella1, Rachel B. Nelson1, Donald E. Elmore1 1Department of Chemistry, Wellesley College This protocol details a method for the quantitative measure of peptide translocation into large unilamellar lipid vesicles. This method also provides information about the rate of membrane translocation and can be used to identify peptides that efficiently and spontaneously cross lipid bilayers.