Biogen Idec View Institution's Website 1 article published in JoVE Neuroscience Derivation of Glial Restricted Precursors from E13 mice André W. Phillips1,2, Sina Falahati1,2, Roshi DeSilva1,3, Irina Shats2, Joel Marx1, Edwin Arauz1, Douglas A. Kerr4, Jeffrey D. Rothstein2,5, Michael V. Johnston1,2,6, Ali Fatemi1,2,6 1Hugo W. Moser Research Institute at Kennedy Krieger, Johns Hopkins University, 2Department of Neurology, Johns Hopkins School of Medicine, 3University of Maryland, 4Experimental Neurology, Biogen Idec, 5The Brain Science Institute, Johns Hopkins School of Medicine, 6Department of Pediatrics, Johns Hopkins School of Medicine This protocol outlines the derivation of Glial Restricted Precursors from fetal spinal cords and maintained in vitro either for transplantation or for the study of oligodendrocytic lineage.