2 articles published in JoVE
Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography Yongyang Huang1, Jinyun Zou1, Mudabbir Badar1, Junchao Liu1, Wentao Shi5, Shunqiang Wang2, Qiongyu Guo3, Xiaofang Wang1, Sarah Kessel4, Leo Li-Ying Chan4, Peter Li4, Yaling Liu2,5, Jean Qiu4, Chao Zhou1,5,6 1Department of Electrical and Computer Engineering, Lehigh University, 2Department of Mechanical Engineering, Lehigh University, 3Department of Biomedical Engineering, Southern University of Science and Technology, 4Department of Technology R&D, Nexcelom Bioscience LLC, 5Department of Bioengineering, Lehigh University, 6Center for Photonics and Nanoelectronics, Lehigh University Optical coherence tomography (OCT), a three-dimensional imaging technology, was used to monitor and characterize the growth kinetics of multicellular tumor spheroids. Precise volumetric quantification of tumor spheroids using a voxel counting approach, and label-free dead tissue detection in the spheroids based on intrinsic optical attenuation contrast, were demonstrated.
Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells Charlotte Berkes1,2, Leo Li-Ying Chan2,3, Alisha Wilkinson1,2,3, Benjamin Paradis2,3 1Department of Biology, Merrimack College, 2Center for Biotechnology and Biomedical Sciences, Merrimack College, 3Department of Technology R&D, Nexcelom Bioscience LLC Here, we demonstrate how image cytometry can be used for quantification of pathogenic fungi in association with host cells in culture. This technique can be used as an alternative to CFU enumeration.