UMass Chan Medical School 3 articles published in JoVE Neuroscience Automated Multimodal Stimulation and Simultaneous Neuronal Recording from Multiple Small Organisms Hamilton White1,2, Vanessa Kamara1, Veronika Gorski1, Molly Busby1, Dirk R. Albrecht1,3 1Department of Biomedical Engineering, Worcester Polytechnic Institute, 2Graduate School of Biomedical Sciences, UMass Chan Medical School, 3Department of Biology and Biotechnology, Worcester Polytechnic Institute We present a method for the flexible chemical and multimodal stimulation and recording of simultaneous neural activity from many Caenorhabditis elegans worms. This method uses microfluidics, open-source hardware and software, and supervised automated data analysis to enable the measurement of neuronal phenomena such as adaptation, temporal inhibition, and stimulus crosstalk. Developmental Biology Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry Aishwarya Swaminathan1, Yung Hwang1, Ashley Winward1, Merav Socolovsky1 1Department of Molecular, Cell and Cancer Biology, UMASS Chan Medical School Here, we describe a novel flow cytometric method for prospective isolation of early burst-forming unit erythroid (BFU-e) and colony-forming unit erythroid (CFU-e) progenitors directly from fresh mouse bone marrow and spleen. This protocol, developed based on single-cell transcriptomic data, is the first to isolate all the tissue's erythroid progenitors with high purity. Bioengineering In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening Prathibha Yarikipati1, Andreas Bergmann1 1Department of Molecular, Cell and Cancer Biology, UMass Chan Medical School Here we present a protocol to express and purify recombinant Drosophila caspases Dronc and Drice, and their use in in vitro cleavage assays.
