UMass Chan Medical School

2 articles published in JoVE

• Developmental Biology

  
  Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry Aishwarya Swaminathan¹, Yung Hwang¹, Ashley Winward¹, Merav Socolovsky^{1 1}Department of Molecular, Cell and Cancer Biology, UMASS Chan Medical School Here, we describe a novel flow cytometric method for prospective isolation of early burst-forming unit erythroid (BFU-e) and colony-forming unit erythroid (CFU-e) progenitors directly from fresh mouse bone marrow and spleen. This protocol, developed based on single-cell transcriptomic data, is the first to isolate all the tissue's erythroid progenitors with high purity.

• Bioengineering

  
  In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening Prathibha Yarikipati¹, Andreas Bergmann^{1 1}Department of Molecular, Cell and Cancer Biology, UMass Chan Medical School Here we present a protocol to express and purify recombinant Drosophila caspases Dronc and Drice, and their use in in vitro cleavage assays.