Boston College View Institution's Website 7 articles published in JoVE Developmental Biology A Drosophila Model to Study Wound-induced Polyploidization Erin C. Bailey*1, Ari S. Dehn*1, Kayla J. Gjelsvik2, Rose Besen-McNally1, Vicki P. Losick1 1Biology Department, Boston College, 2Graduate School of Biomedical Sciences and Engineering and Kathryn W. Davis Center for Regenerative Biology and Medicine, MDI Biological Laboratory, University of Maine Wound-induced polyploidization is a conserved tissue repair strategy where cells grow in size instead of dividing to compensate for cell loss. Here is a detailed protocol on how to use the fruit fly as a model to measure ploidy and its genetic regulation in epithelial wound repair. Biochemistry Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants Hyelee Lee1, Shih-Yuan Liu1 1Department of Chemistry, Boston College A protocol for the synthesis of 1,2-azaborines and the preparation of their protein complexes with T4 lysozyme mutants is presented. Biology RNAi Trigger Delivery into Anopheles gambiae Pupae Kimberly Regna1, Rachel M. Harrison1, Shannon A. Heyse1, Thomas C. Chiles1, Kristin Michel2, Marc A. T. Muskavitch1,3 1Biology Department, Boston College, 2Division of Biology, Kansas State University, 3Discovery Research, Biogen RNA interference (RNAi) is an extremely valuable tool for uncovering gene function. However, the ability to target genes using RNAi during pre-adult stages is limited in the major human malaria vector Anopheles gambiae. We describe an RNAi protocol to reduce gene function via direct injection during pupal development. Biology Using plusTipTracker Software to Measure Microtubule Dynamics in Xenopus laevis Growth Cones Alina Stout1, Salvatore D'Amico1, Tiffany Enzenbacher1, Patrick Ebbert1, Laura Anne Lowery1 1Department of Biology, Boston College The MATLAB-based, open source software package, plusTipTracker, can be used to analyze image series of fluorescently-labeled +TIPs to quantify microtubule dynamics. Behavior Eye Tracking, Cortisol, and a Sleep vs. Wake Consolidation Delay: Combining Methods to Uncover an Interactive Effect of Sleep and Cortisol on Memory Kelly A. Bennion1, Katherine R. Mickley Steinmetz2, Elizabeth A. Kensinger1, Jessica D. Payne3 1Department of Psychology, Boston College, 2Department of Psychology, Wofford College, 3Department of Psychology, University of Notre Dame We present a protocol used to discover an interactive effect between sleep and cortisol on memory consolidation, particularly for negative arousing images. Specifically, the experimental design utilizes eye tracking, salivary cortisol analysis, and behavioral memory testing – methods that can be used with both healthy and clinical participants. Immunology and Infection A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants Bradley I. Coleman1, Marc-Jan Gubbels1 1Department of Biology, Boston College Forward genetics is a powerful method to unravel the molecular level of how Toxoplasma egresses from its host cell. Protocols are provided to chemically mutagenize parasites, enrich for mutants with defects in induced egress, and validate the phenotype of cloned mutants. Neuroscience High Density Event-related Potential Data Acquisition in Cognitive Neuroscience Scott D. Slotnick1 1Department of Psychology, Boston College Event-related potential (ERP) recording is under utilized in Cognitive Neuroscience because data acquisition techniques are not readily available and this method often has poor spatial resolution. To foster the increased use of ERPs in Cognitive Neuroscience, the present article details key techniques involved in high density ERP data acquisition.