3 articles published in JoVE
Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae Meagan Jezek1, Alison Jacques1, Deepika Jaiswal1, Erin M. Green1 1Department of Biological Sciences, University of Maryland Here, we describe a protocol for chromatin immunoprecipitation of modified histones from the budding yeast Saccharomyces cerevisiae. Immunoprecipitated DNA is subsequently used for quantitative PCR to interrogate the abundance and localization of histone post-translational modifications throughout the genome.
Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo Rebecca J. McFarland*1, Sharlene P. Brown*1, Eudorah Vital1, Jonathan M. Werner1, Rachel M. Brewster1 1Department of Biological Sciences, University of Maryland, Baltimore County Immunolabeling methods to analyze distinct populations of microtubules in the developing zebrafish brain are described here, which are broadly applicable to other tissues. The first protocol outlines an optimized method for immunolabeling stable and dynamic microtubules. The second protocol provides a method to image and quantify nascent microtubules specifically.
Labeling and Imaging Cells in the Zebrafish Hindbrain Pradeepa Jayachandran1, Elim Hong2, Rachel Brewster1 1Department of Biological Sciences, University of Maryland, Baltimore County, 2Center for Neuroscience, Children's National Medical Center Key to understanding the morphogenetic processes that shape the early embryo is the ability to image cells at high resolution. We describe here a technique for labeling single cells or small clusters of cells in whole zebrafish embryos with membrane-targeted Green Fluorescent Protein.