National Eye Institute/National Institutes of Health 1 article published in JoVE Medicine A Step by Step Protocol for Subretinal Surgery in Rabbits Sami Al-Nawaiseh1, Fabian Thieltges1, Zengping Liu1,2, Claudine Strack1, Ralf Brinken1, Norbert Braun3, Marc Wolschendorf3, Arvydas Maminishkis5, Nicole Eter4, Boris V. Stanzel1,6 1Department of Ophthalmology, University of Bonn, 2Department of Ophthalmology, National University of Singapore, 3Geuder AG, 4Department of Ophthalmology, University of Münster, 5Section on Epithelial and Retinal Physiology and Disease, National Eye Institute/National Institutes of Health, 6Surgical Retina Department, Singapore National Eye Centre Retinal pigment epithelium (RPE) replacement strategies and gene-based therapy are considered for several retinal degenerative conditions. For clinical translation, large eye animal models are required to study surgical techniques applicable in patients. Here we present a rabbit model for subretinal surgery geared towards RPE transplantation, which is versatile and cost-efficient.
