3 articles published in JoVE
Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice David E. Maridas1, Elizabeth Rendina-Ruedy1, Phuong T. Le1, Clifford J. Rosen1 1Maine Medical Center Research Institute In this article, we present methods to isolate and differentiate bone marrow stromal cells and hematopoietic stem cells from mouse long bones. Two different protocols are presented yielding different cell populations suitable for expansion and differentiation into osteoblasts, adipocytes, and osteoclasts.
Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix Matthew K. Stephenson1, Sean Lenihan2,3, Roman Covarrubias2,3, Ryan M. Huttinger2,3, Richard J. Gumina2,3, Douglas B. Sawyer4, Cristi L. Galindo2,3 1Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, 2Department of Medicine, Vanderbilt University Medical Center, 3Division of Cardiovascular Medicine, Vanderbilt University Medical Center, 4Cardiovascular Institute, Maine Medical Center Shown here is a method for visualizing extracellular matrix ultrastructure in decellularized cardiac tissues.
Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney Aaron C. Brown1, Ulrika Blank2, Derek C. Adams1, Michele J. Karolak1, Jennifer L. Fetting1, Beth L. Hill1, Leif Oxburgh1 1Department of Molecular Medicine, Maine Medical Center Research Institute, 2Molecular Medicine and Gene Therapy, Lund University Hospital In this report we describe a method for the isolation and culture of the progenitor cell niche from the embryonic mouse kidney that can be used to study signaling pathways regulating stem/progenitor cells of the developing kidney. These cultured cells are highly accessible to small molecule and recombinant protein treatment, and importantly also to viral transduction, which allows efficient manipulation of candidate pathways.