3 articles published in JoVE
Analysis of Histone Antibody Specificity with Peptide Microarrays Evan M. Cornett1, Bradley M. Dickson1, Scott B. Rothbart1 1Center for Epigenetics, Van Andel Research Institute This manuscript describes methods for applying peptide microarray technology to specificity profiling of antibodies that recognize histones and their post-translational modifications.
Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples Tyler Rhinesmith1, Bryan A. Killinger2, Akhil Sharma3, Anna Moszczynska3 1Physiology, Michigan State University, 2Center for Neurodegenerative Science Van Andel Institute, 3Pharmaceutical Sciences, Wayne State University A method for stabilizing and separating native protein complexes from unmodified tissue lysate using an amine-reactive protein cross-linker coupled to a novel two-dimensional polyacrylamide gel electrophoresis (PAGE) system is presented.
Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples Chen Lu1, Joshua L. Wonsidler1, Jianwei Li2, Yanming Du1, Timothy Block3, Brian Haab4, Songming Chen5 1Institute for Hepatitis and Virus Research, 2Department of Microbiology and Immunology, Thomas Jefferson University, 3Drexel University College of Medicine, 4Van Andel Research Institute, 5Institute for Hepatitis and Virus Research, Serome Biosciences Inc. In this study, we describe an improved protocol for a multiplexed high-throughput antibody microarray with lectin detection method that can be used in glycosylation profiling of specific proteins. This protocol features new reliable reagents and significantly reduces the time, cost, and lab equipment requirements as compared to the previous procedure.