2 articles published in JoVE
Analysis of Histone Antibody Specificity with Peptide Microarrays Evan M. Cornett1, Bradley M. Dickson1, Scott B. Rothbart1 1Center for Epigenetics, Van Andel Research Institute This manuscript describes methods for applying peptide microarray technology to specificity profiling of antibodies that recognize histones and their post-translational modifications.
Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples Chen Lu1, Joshua L. Wonsidler1, Jianwei Li2, Yanming Du1, Timothy Block3, Brian Haab4, Songming Chen5 1Institute for Hepatitis and Virus Research, 2Department of Microbiology and Immunology, Thomas Jefferson University, 3Drexel University College of Medicine, 4Van Andel Research Institute, 5Institute for Hepatitis and Virus Research, Serome Biosciences Inc. In this study, we describe an improved protocol for a multiplexed high-throughput antibody microarray with lectin detection method that can be used in glycosylation profiling of specific proteins. This protocol features new reliable reagents and significantly reduces the time, cost, and lab equipment requirements as compared to the previous procedure.