2 articles published in JoVE
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens Saman Saeidi*1, Michael R. McKain*1,2, Elizabeth A. Kellogg1 1Donald Danforth Plant Science Center, 2Department of Biological Sciences, The University of Alabama This article demonstrates a detailed protocol for DNA isolation and high-throughput sequencing library construction from herbarium material including rescue of exceptionally poor-quality DNA.
Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins Kazi T. Islam1, Dilip M. Shah1, Kaoutar El-Mounadi2 1Donald Danforth Plant Science Center, 2Department of Biology, Kutztown University of Pennsylvania Plant defensins play an important role in plant defense against pathogens. For effective use of these antifungal peptides as antifungal agents, understanding their modes of action (MOA) is critical. Here, a live-cell imaging method is described to study critical aspects of the MOA of these peptides.