University of Missouri 8 articles published in JoVE Bioengineering Troubleshooting and Quality Assurance in Hyperpolarized Xenon Magnetic Resonance Imaging: Tools for High-Quality Image Acquisition Ummul Afia Shammi1, Gabriela María Garcίa Delgado2, Robert Thomen2,3 1Roy Blunt NextGen Precision Health Center, University of Missouri, 2Chemical and Biomedical Engineering, University of Missouri, 3Radiology, School of Medicine, University of Missouri Here, we present a protocol for obtaining high-quality hyperpolarized xenon-129 magnetic resonance images, covering hardware, software, data acquisition, sequence selection, data management, k-space utilization, and noise analysis. Behavior Loneliness Assuaged: Eye-Tracking an Audience Watching Barrage Videos Guangyao Chen1, Shuhua Zhou2 1School of Journalism & Communication/National Media & Experimental Teaching Center, Jinan University, 2School of Journalism, University of Missouri The study proposes an activation-match model to study how loneliness is mitigated when a lonely audience watches barrage videos of rational and emotional appeals. The protocol uses eye tracking to document duration and fixation, accounting for the degree of satisfaction when emotional needs are appeased by content and barrage. Biochemistry Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation Emily A. Brown1,2, Steven C. Neier3,4, Claudia Neuhauser5, Adam G. Schrum6,7,8, Stephen E.P. Smith1,2,9 1 Quantitative Multiplex Immunoprecipitation (QMI) uses flow cytometry for sensitive detection of differences in the abundance of targeted protein-protein interactions between two samples. QMI can be performed using a small amount of biomaterial, does not require genetically engineered tags, and can be adapted for any previously defined protein interaction network. Engineering Precision Milling of Carbon Nanotube Forests Using Low Pressure Scanning Electron Microscopy Josef Brown1, Benjamin F. Davis1, Matthew R. Maschmann1 1Department of Mechanical & Aerospace Engineering, University of Missouri Low pressure scanning electron microscopy in a water vapor ambient is used to machine nanoscale to microscale features in carbon nanotube forests. Immunology and Infection Reliable and High Efficiency Extraction of Kidney Immune Cells Ravi Nistala1, Alex Meuth2, Cassandra Smith3, Aroor Annayya3 1Division of Nephrology, Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital, 2Division of Biomedical Sciences, Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital, 3Division of Endocrinology, Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital Techniques that are reliable and efficient for the isolation of kidney immune cells are needed for downstream applications. This requires surface antibody labeling of a small number of kidney immune cells. Herein, we describe a concise method for isolation of kidney immune cells that seemingly achieves this goal. Medicine Photothrombosis-induced Focal Ischemia as a Model of Spinal Cord Injury in Mice Hailong Li*1, Gourav Roy Choudhury*1, Nannan Zhang1, Shinghua Ding1 1Department of Bioengineering, Dalton Cardiovascular Research Center, University of Missouri Photothrombosis is a minimally invasive and highly reproducible procedure to induce focal ischemia in the spinal cord and serves as a model of spinal cord injury in mice. Medicine Adapting Human Videofluoroscopic Swallow Study Methods to Detect and Characterize Dysphagia in Murine Disease Models Teresa E. Lever1, Sabrina M. Braun2, Ryan T. Brooks2, Rebecca A. Harris2, Loren L. Littrell2, Ryan M. Neff3, Cameron J. Hinkel3, Mitchell J. Allen1, Mollie A. Ulsas2 1Department of Otolaryngology - Head and Neck Surgery, University of Missouri, 2Department of Communication Science and Disorders, University of Missouri, 3Department of Medicine, University of Missouri This study successfully adapted human videofluoroscopic swallowing study (VFSS) methods for use with murine disease models for the purpose of facilitating translational dysphagia research. Behavior Barnes Maze Testing Strategies with Small and Large Rodent Models Cheryl S. Rosenfeld*1, Sherry A. Ferguson*2 1Biomedical Sciences and Bond Life Sciences Center, University of Missouri, 2Division of Neurotoxicology, National Center for Toxicological Research, Food and Drug Administration The dry-land Barnes maze is widely used to measure spatial navigation ability in response to mildly aversive stimuli. Over consecutive days, performance (e.g. latency to locate escape cage) of control subjects improves, indicative of normal learning and memory. Differences between rats and mice necessitate apparatus and methodology changes that are detailed here.
