3 articles published in JoVE
Behavioral Assessment of Hearing in 2 to 4 Year-old Children: A Two-interval, Observer-based Procedure Using Conditioned Play-based Responses Angela Yarnell Bonino1,2, Lori J. Leibold3 1Department of Allied Health Sciences, The University of North Carolina at Chapel Hill, 2Department of Speech, Language, and Hearing Sciences, University of Colorado Boulder, 3Center for Hearing Research, Boys Town National Research Hospital This paper describes a procedure for measuring hearing sensitivity in 2 to 4 year-old children. Children are trained to perform play-based responses when they hear a target signal. Thresholds are then estimated in a two-interval, two-alternative forced-choice paradigm, based on observations of the child's behaviors.
Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis Cortney C. Winkle1, Christopher C. Hanlin2, Stephanie L. Gupton1,2 1Curriculum in Neurobiology, The University of North Carolina at Chapel Hill, 2Department of Cell Biology and Physiology, The University of North Carolina at Chapel Hill Light microscopy techniques coupled with biochemical assays elucidate the involvement of SNARE-mediated exocytosis in netrin-dependent axon branching. This combination of techniques permits identification of molecular mechanisms controlling axon branching and cell shape change.
Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy Johnny L. Carson1 1Department of Pediatrics, Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill Basic techniques and refinements of freeze-fracture processing of biological specimens and nanomaterials for examination by transmission electron microscopy are described. This technique is a preferred method for revealing ultrastructural features and specializations of biological membranes and for obtaining ultrastructural level dimensional and spatial data in materials sciences and nanotechnology products.