LifeSciences, LifeNet Health 1 article published in JoVE Biology An All-Human Hepatic Culture System for Drug Development Applications Justin J. Odanga1, Elena Gianulis2, Lindsey Whaley3, Edward L. LeCluyse4, Sharon Presnell1, Jessica R. Weaver1 1Institute of Regenerative Medicine, LifeNet Health, 2Biologics Division, LifeNet Health, 3LifeSciences Division, LifeNet Health, 4Research and Development, LifeSciences, LifeNet Health Recent technical advances enabled the scaled production of an in vitro platform for drug metabolism and toxicity applications. An all-human 2D+ hepatic system (TV2D+) provides physiologically relevant results using traditional two-dimensional culture methods. This protocol will support end-users in the setup, maintenance, and application of the system.