3 articles published in JoVE
Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion Salvatore Coniglio1, Ian Miller2, Marc Symons3, Jeffrey E. Segall4 1New Jersey Center for Science, Technology and Mathematics, Kean University, 2Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, 3The Feinstein Institute for Medical Research at North Shore-LIJ, 4Department of Anatomy and Structural Biology, Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine Understanding the malignant behavior of cancer requires creating accurate models of how tumor cells interact with components of the tumor microenvironment, such as macrophages. Here we describe two methods to study glioblastoma cell interaction with tumor associated macrophages and microglia where the effect on glioblastoma invasion is assessed.
Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages Farshad Tamari1, Joanna Tychowski2, Laura Lorentzen3 1Department of Biological Sciences, Kingsborough Community College, 2Institute for Cellular and Molecular Biology, University of Texas at Austin, 3New Jersey Center for Science, Technology and Mathematics, Kean University We set out to determine tolerable concentrations of three fatty acids (oleic, linoleic and linolenic acids) and cholera toxin that did not significantly and adversely affect cell survival by solubilizing the fatty acids and the toxin and using them in cell survival assays.
Trajectory Data Analyses for Pedestrian Space-time Activity Study Feng Qi1, Fei Du2 1School of Environmental and Life Sciences, Kean University, 2Department of Geography, University of Wisconsin-Madison A suite of spatiotemporal processing methods are presented to analyze human trajectory data, such as that collected using a GPS device, for the purpose of modeling pedestrian space-time activities.