Rutgers New Jersey Medical School 6 articles published in JoVE Cancer Research Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line Minh Ma1,2, Saiaditya Badeti1, James K. Kim1, Dongfang Liu1,3 1Department of Pathology, Immunology and Laboratory Medicine, Rutgers-New Jersey Medical School, 2Department of Microbiology, Biochemistry & Molecular Genetics, Public Health Research Institute Center, New Jersey Medical School, Rutgers University, 3Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey Here, we present a method to expand peripheral blood natural killer (PBNK), NK cells from liver tissues, and chimeric antigen receptor (CAR)-NK cells derived from peripheral blood mononuclear cells (PBMCs) or cord blood (CB). This protocol demonstrates the expansion of NK and CAR-NK cells using 221-mIL-21 feeder cells in addition to the optimized purity of expanded NK cells. Immunology and Infection Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine Luo Jia1, Karen L. Edelblum1 1Center for Immunity and Inflammation, Department of Pathology, Immunology and Laboratory Medicine, Rutgers New Jersey Medical School We describe a method to visualize GFP-labeled γδ IELs using intravital imaging of murine small intestine by inverted spinning disk confocal microscopy. This technique enables the tracking of live cells within the mucosa for up to 4 h and can be used to investigate a variety of intestinal immune-epithelial interactions. Bioengineering A Versatile Method of Patterning Proteins and Cells Anil B. Shrirao*1, Frank H. Kung*2, Derek Yip3, Bonnie L. Firestein2, Cheul H, Cho3, Ellen Townes-Anderson4 1Department of Biomedical Engineering, Rutgers University, 2Department of Cell Biology and Neuroscience, Rutgers University, 3Department of Biomedical Engineering, New Jersey Institute of Technology, 4Department of Pharmacology, Physiology, and Neuroscience, Rutgers New Jersey Medical School This report describes a simple, easy to perform technique, using low pressure vacuum, to fill microfluidic channels with cells and substrates for biological research. Neuroscience Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers Bogumila Swietek1, Akshay Gupta2, Archana Proddutur2, Vijayalakshmi Santhakumar2 1Graduate School of Biomedical Sciences, Rutgers New Jersey Medical School, 2Department of Pharmacology, Physiology and Neuroscience, Rutgers New Jersey Medical School This protocol presents a method for the morphological recovery of neurons patched during electrophysiological recordings using biocytin filling and subsequent immunohistochemical postprocessing. We show that thick biocytin-filled sections that were stained and coverslipped can be restained with a second primary antibody days or months later. Bioengineering Culturing Mouse Cardiac Valves in the Miniature Tissue Culture System Boudewijn P.T. Kruithof1, Samuel C. Lieber2, Marianna Kruithof-de Julio3, Vincian Gaussin4, Marie José Goumans1 1Department of Molecular Cell Biology, Leiden University Medical Center, 2Department of Engineering Technology, New Jersey Institute of Technology, 3Department of Urology, Leiden University Medical Center, 4Cardiovascular Research Institute, Department of Cell Biology and Molecular Medicine, Rutgers New Jersey Medical School Here, we present an ex vivo flow model in which murine cardiac valves can be cultured allowing the study of the biology of the valve. Medicine An In Vitro Dormancy Model of Estrogen-sensitive Breast Cancer in the Bone Marrow: A Tool for Molecular Mechanism Studies and Hypothesis Generation Samir Tivari1, Reju Korah1, Michael Lindy1, Robert Wieder1 1Department of Medicine and New Jersey Medical School Cancer Center, Rutgers New Jersey Medical School We developed an in vitro model of dormancy in the bone marrow for estrogen-sensitive breast cancer cells. The goal of this protocol is to demonstrate use of the model for the study of the molecular and cellular biology of dormancy and for generation of hypotheses for subsequent testing in vivo.
