2 articles published in JoVE
An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells Lauren S. Sherman1,2, Alexandra Condé-Green3, Yahaira Naaldijk1, Edward S. Lee3, Pranela Rameshwar1,2 1Department of Medicine, Division of Hematology/Oncology, New Jersey Medical School, Rutgers Biomedical and Health Sciences, 2Rutgers School of Graduate Studies at New Jersey Medical School, Rutgers Biomedical and Health Sciences, 3Department of Surgery, Division of Plastic Surgery, New Jersey Medical School, Rutgers Biomedical and Health Sciences This protocol provides an enzyme-free method for isolating mesenchymal stem cells from abdominoplasty and lipoaspirate samples using an explant method. The absence of harsh enzymes or centrifugation steps provides for clinically relevant stem cells that can be used for studies in vitro or transferred back to the clinic.
Stochastic Noise Application for the Assessment of Medial Vestibular Nucleus Neuron Sensitivity In Vitro Sebastian P. Stefani1,2, Paul P. Breen3, Jorge M. Serrador4, Aaron J. Camp1,2 1Discipline of Physiology, Sydney Medical School, University of Sydney, 2Bosch Institute, Sydney Medical School, University of Sydney, 3The MARCS Institute, Western Sydney University, 4Department of Pharmacology, Physiology & Neuroscience, Rutgers Biomedical and Health Sciences Galvanic vestibular stimulation in humans exhibits improvements in vestibular function. However, it is unknown how these effects occur. Here, we describe how to apply sinusoidal and stochastic electrical noise and evaluate appropriate stimulus amplitudes in individual medial vestibular nucleus neurons in the C57BL/6 mouse.