Los Alamos National Laboratory

2 articles published in JoVE

• Chemistry

  
  Ligand-Mediated Nucleation and Growth of Palladium Metal Nanoparticles Saeed Mozaffari*¹, Wenhui Li*¹, Coogan Thompson¹, Sergei Ivanov², Soenke Seifert³, Byeongdu Lee⁴, Libor Kovarik⁵, Ayman M. Karim^{1 1}Department of Chemical Engineering, Virginia Polytechnic Institute and State University, ²Center for Integrated Nanotechnologies, Los Alamos National Laboratory, ³Advanced Photon Source, Argonne National Laboratory, ⁴X-ray Science Division, Argonne National Laboratory, ⁵Environmental Molecular Science Laboratory, Pacific Northwest National Laboratory The main goal of this work is to elucidate the role of capping agents in regulating the size of palladium nanoparticles by combining in situ small angle x-ray scattering (SAXS) and ligand-based kinetic modeling.

• Biology

  
  Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution Miriam Cohen¹, Nissi M. Varki¹, Mark D. Jankowski², Pascal Gagneux^{1 1}Department of Cellular and Molecular Medicine, University of California, San Diego, ²Biosecurity and Public Health, Los Alamos National Laboratory Unfixed frozen tissue samples embedded in Optimal Cutting Temperature medium (OCT) can be used to study natural distribution and glycosylation of secreted mucus. In this approach tissue processing is minimal and the natural presentation of glycolipids, mucins and glycan-epitopes is preserved. Tissue sections can be analyzed by immunohistochemistry using fluorescence or chromogenic detection.