2 articles published in JoVE
Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies Neil D. Sanscrainte1, Christy M. Waits2,3, Christopher J. Geden1, Alden S. Estep1,2, James J. Becnel1 1USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology, 2CMAVE Detachment, Navy Entomology Center of Excellence, 3Lovelace Respiratory Research Institute This protocol describes a microinjection methodology that we have standardized and used for several years to deliver specific quantities of nucleic acids directly to the hemolymph of mosquitoes and house flies. This protocol results in minimal injection mortality and allows dose correlated measurements of fecundity.
Automated Measurement of Pulmonary Emphysema and Small Airway Remodeling in Cigarette Smoke-exposed Mice Maria E. Laucho-Contreras1, Katherine L. Taylor1, Ravi Mahadeva2, Steve S. Boukedes3, Caroline A. Owen1,4 1 The goal of this protocol is to provide automated methods to quantify chronic lung pathologies in a murine model of COPD. The protocol includes exposing mice to cigarette smoke (CS), measuring pulmonary function, inflating the lungs, and using morphometry methods to measure emphysema and small airway remodeling in mice.