3 articles published in JoVE
Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches Magdia De Jesus1, Sarita Ahlawat1, Nicholas J. Mantis1 1Division of Infectious Diseases, New York State Department of Health There is an increasing interest in understanding the immunological functions of specific subpopulations of cells in Peyer's patches (PPs), the primary inductive sites of gut-associated lymphoid tissues. Here we outline parallel protocols for preparing PP single cell preparations for flow cytometric analysis and PP cryosections for immunostaining.
Recording Human Electrocorticographic (ECoG) Signals for Neuroscientific Research and Real-time Functional Cortical Mapping N. Jeremy Hill1, Disha Gupta1,2, Peter Brunner1,2, Aysegul Gunduz1,2, Matthew A. Adamo3, Anthony Ritaccio2, Gerwin Schalk1,2,4,5,6,7 1Wadsworth Center, New York State Department of Health, 2Department of Neurology, Albany Medical College, 3Department of Neurosurgery, Albany Medical College, 4Department of Neurosurgery, Washington University, 5Department of Biomed. Eng., Rensselaer Polytechnic Institute, 6Department of Biomed. Sci., State University of New York at Albany, 7Department of Elec. and Comp. Eng., University of Texas at El Paso We present a method for collecting electrocorticographic signals for research purposes from humans who are undergoing invasive epilepsy monitoring. We show how to use the BCI2000 software platform for data collection, signal processing and stimulus presentation. Specifically, we demonstrate SIGFRIED, a BCI2000-based tool for real-time functional brain mapping.
Using an EEG-Based Brain-Computer Interface for Virtual Cursor Movement with BCI2000 J. Adam Wilson1, Gerwin Schalk2, Léo M. Walton1, Justin C. Williams1 1Department of Biomedical Engineering, University of Wisconsin-Madison, 2Wadsworth Center, New York State Dept. of Health In this video, we demonstrate the steps required to run a brain-computer interface experiment, including setting up the EEG cap, calibrating the system, and training the user to move a cursor in two dimensions using imagined movements.