2 articles published in JoVE
Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes Joseph D. Tario Jr.1, Kristen Humphrey1, Andrew D. Bantly2, Katharine A. Muirhead3, Jonni S. Moore4, Paul K. Wallace1 1Department of Flow and Image Cytometry, Roswell Park Cancer Institute, 2Flow Cytometry & Cell Sorting Resource Laboratory, University of Pennsylvania, 3SciGro, Inc., 4Department of Pathology and Laboratory Medicine, University of Pennsylvania Successful use of cell tracking dyes to monitor immune cell function and proliferation involves several critical steps. We describe methods for: 1) obtaining bright, uniform, reproducible label-ing with membrane dyes; 2) selecting fluorochromes and data acquisition conditions; and 3) choosing a model to quantify cell proliferation based on dye dilution.
Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models Wei Han1, Hui Li1, Brahm H. Segal2,3, Timothy S. Blackwell1 1Department of Medicine, Vanderbilt University School of Medicine, 2Departments of Medicine and Immunology, Roswell Park Cancer Institute, 3Department of Medicine, University at Buffalo School of Medicine NADPH oxidase is the major source of reactive oxygen species (ROS) in phagocytes. Because of the ephemeral nature of ROS, it is difficult to measure and monitor ROS levels in living animals. A minimally invasive method for serial quantification of ROS in living mice is described.