Roswell Park Comprehensive Cancer Center 4 articles published in JoVE Cancer Research Ex Vivo Organoid Model of Adenovirus-Cre Mediated Gene Deletions in Mouse Urothelial Cells Dongbo Xu1, Li Wang1, Kyle Wieczorek1, Yanqing Wang2, Xiaojing Zhang2, David W. Goodrich2, Qiang Li1,2 1Department of Urology, Roswell Park Comprehensive Cancer Center, 2Department of Pharmacology and Therapeutics, Roswell Park Comprehensive Cancer Center This protocol describes the process of the generation and characterization of mouse urothelial organoids harboring deletions in genes of interest. The methods include harvesting mouse urothelial cells, ex vivo transduction with adenovirus driving Cre expression with a CMV promoter, and in vitro as well as in vivo characterization. Cancer Research A Murine Ommaya Xenograft Model to Study Direct-Targeted Therapy of Leptomeningeal Disease Vincent Law1,2, Margi Baldwin3, Ganesan Ramamoorthi4, Krithika Kodumudi4, Nam Tran1, Inna Smalley5, Derek Duckett6, Pawel Kalinski7, Brian Czerniecki4, Keiran S. M. Smalley2, Peter A. Forsyth1,2 1Department of Neuro-Oncology, H. Lee Moffitt Cancer Center & Research Institute, 2Department of Tumor Biology, H. Lee Moffitt Cancer Center & Research Institute, 3Department of Comparative Medicine, University of South Florida, 4Department of Breast Oncology, H. Lee Moffitt Cancer Center & Research Institute, 5Department of Cancer Physiology, H. Lee Moffitt Cancer Center & Research Institute, 6Department of Drug Discovery, H. Lee Moffitt Cancer Center & Research Institute, 7Department of Medical Oncology, Roswell Park Comprehensive Cancer Center Here, we describe a murine xenograft model that functionally resembles an Ommaya reservoir in patients. We developed the Murine Ommaya to study novel therapeutics for the universally fatal leptomeningeal disease. Genetics Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer Kristine M. Wadosky1, Yanqing Wang1, Xiaojing Zhang1, David W. Goodrich1 1Department of Pharmacology & Therapeutics, Roswell Park Comprehensive Cancer Center We show a method for necropsy and dissection of mouse prostate cancer models, focusing on prostate tumor dissection. A step-by-step protocol for generation of mouse prostate tumor organoids is also presented. Medicine Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy Eriko Katsuta1, Masanori Oshi1, Omar M. Rashid2,3,4,5, Kazuaki Takabe1,6,7,8,9,10 1Breast Surgery, Department of Surgical Oncology, Roswell Park Comprehensive Cancer Center, 2Holy Cross Hospital Michael and Dianne Bienes Comprehensive Cancer Center, 3Department of Surgery, Massachusetts General Hospital, 4Department of Surgery, University of Miami Miller School of Medicine, 5Department of Surgery, Nova Southeastern University School of Medicine, 6Department of Surgery, University at Buffalo Jacobs School of Medicine and Biomedical Sciences, 7Department of Breast Surgery and Oncology, Tokyo Medical University, 8Department of Surgery, Yokohama City University, 9Department of Surgery, Niigata University Graduate School of Medical and Dental Sciences, 10Department of Surgery, Fukushima Medical University We introduce a murine orthotopic breast cancer model and radical mastectomy model with bioluminescence technology to quantify the tumor burden to mimic human breast cancer progression.
