3 articles published in JoVE
Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain Emily D. Geyer*1, Prithvi A. Shetty*1, Christopher J. Suozzi*1, David Z. Allen*1,2, Pamela P. Benavidez*1,2, Joseph Liu*1,3, Charles N. Hollis1, Greg A. Gerhardt4, Jorge E. Quintero4, Jason J. Burmeister4, Emmett E. Whitaker1,3 1Department of Anesthesiology, Ohio State University College of Medicine, 2Medical Student Research Program, Ohio State University College of Medicine, 3 This study explores the novel use of enzyme-based microelectrode array (MEA) technology to monitor in vivo neurotransmitter activity in piglets. The hypothesis was that glutamate dysregulation contributes to the mechanism of anesthetic neurotoxicity. Here, we present a protocol to adapt MEA technology to study the mechanism of anesthesia-induced neurotoxicity.
Assembly and Purification of Prototype Foamy Virus Intasomes Randi M. Mackler1, Miguel A. Lopez Jr.1, Kristine E. Yoder1 1Department of Cancer Biology and Genetics, The Ohio State University College of Medicine Recombinant retroviral integrase and DNA oligomers mimicking viral DNA ends can form an enzymatically active complex known as an intasome. Intasomes may be used for biochemical, structural, and kinetic studies. This protocol details how to assemble and purify prototype foamy virus intasomes.
Use of a Piglet Model for the Study of Anesthetic-induced Developmental Neurotoxicity (AIDN): A Translational Neuroscience Approach Emmett E. Whitaker1,2, Christopher Z. Zheng1, Bruno Bissonnette1,2,3, Andrew D. Miller4, Tanner L. Koppert1,2, Joseph D. Tobias1,2, Christopher R. Pierson5,6, Fedias L. Christofi1 1Department of Anesthesiology, Ohio State University College of Medicine, 2 Anesthesia-induced developmental neurotoxicity (AIDN) research has focused on rodents, which are not broadly applicable to humans. Non-human primate models are more relevant, but are cost-prohibitive and difficult to use for experimentation. The piglet, in contrast, is a clinically relevant, practical animal model ideal for the study of anesthetic neurotoxicity.