1 article published in JoVE
Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis Jonathan James Caguiat1 1Department of Biological Sciences, Center for Applied Chemical Biology, Youngstown State University Enterobacter sp. YSU grows in glucose minimal salts medium. Auxotrophs are generated by transforming it with a transposome which randomly inserts itself into the host genome. Mutants are found by replica plating from complex medium to minimal medium. Interrupted genes are identified by gene rescue and sequencing.