2 articles published in JoVE
Comprehensive Autopsy Program for Individuals with Multiple Sclerosis Ranjan Dutta*1, Kedar R. Mahajan*1,3, Kunio Nakamura*3, Daniel Ontaneda*2, Jacqueline Chen1, Christina Volsko1, Jessica Dudman1, Emilie Christie1, Jordon Dunham1, Robert J. Fox3, Bruce D. Trapp1 1Department of Neurosciences, Lerner Research Institute, Cleveland Clinic, 2Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, 3Mellen Center for Treatment and Research in Multiple Sclerosis, Neurological Institute, Cleveland Clinic Multiple sclerosis is an inflammatory demyelinating disease with no cure. Analysis of brain tissue provides important clues to understanding the pathogenesis of the disease. Here we discuss the methodology and downstream analysis of MS brain tissue collected through a unique rapid autopsy program in operation at the Cleveland Clinic.
Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments Jing Hao1, Anthony Magnelli2, Andrew Godley2, Jennifer S. Yu1,2 1Department of Cancer Biology, Cleveland Clinic, 2Department of Radiation Oncology, Cleveland Clinic Clinical linear accelerators can be used to determine biologic effects of a wide range of dose rates on cancer cells. We discuss how to set up a linear accelerator for cell-based assays and assays for cancer stem-like cells grown as tumorspheres in suspension and cell lines grown as adherent cultures.