2 articles published in JoVE
Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton Steven Robbins*1, Jisha Jacob*1, Xinxin Lu1, Mary Ann Moran2, Xiaozhen Mou1 1Biological Sciences, Kent State University, 2Marine Sciences, University of Georgia (UGA) Environmental bacterioplankton are incubated with a model dissolved organic carbon (DOC) compound and a DNA labeling reagent, bromodeoxyuridine (BrdU). Afterward, DOC-degrading cells are separated from the bulk community based on their elevated BrdU incorporation using fluorescence activated cell sorting (FACS). These cells are then identified by subsequent molecular analyses.
Mouse Model of Middle Cerebral Artery Occlusion Terrance Chiang1, Robert O. Messing1, Wen-Hai Chou1,2 1Department of Neurology, Ernest Gallo Clinic and Research Center, University of California, San Francisco, 2Department of Biological Sciences, Kent State University We demonstrate in the video a method for producing a middle cerebral artery occlusion in adult mice using an intraluminal monofilament. We also show how to evaluate the extent of cerebral infarction by 2,3,5-triphenyltetrazolium chloride (TTC) staining.