University of Oklahoma, Norman

9 articles published in JoVE

• Environment

  
  Simulating Temperature in a Soil Incubation Experiment Jianwei Li¹, Precious Areeveso¹, Xuehan Wang¹, Siyang Jian^1,2, Lahiru Gamage^{1 1}Department of Agricultural and Environmental Sciences, Tennessee State University, ²Department of Plant Biology and Microbiology, University of Oklahoma, Norman Laboratory soil warming experiments usually employ two or more constant temperatures in multiple chambers. By presenting a sophisticated environmental chamber, we provide an accurate temperature control method to imitate the magnitude and amplitude of in situ soil temperature and improve the experimental design of soil incubation studies.

• Immunology and Infection

  
  Chemical Cartography Approaches to Study Trypanosomatid Infection Danya A. Dean*^1,2, Jacob J. Haffner*^2,3, Mitchelle Katemauswa¹, Laura-Isobel McCall^{1,2,4 1}Department of Chemistry and Biochemistry, University of Oklahoma, Norman, ²Laboratories of Molecular Anthropology and Microbiome Research, University of Oklahoma, Norman, ³Department of Anthropology, University of Oklahoma, Norman, ⁴Department of Microbiology and Plant Biology, University of Oklahoma, Norman This protocol describes the steps to generate a 3D model of metabolite distribution during trypanosomatid infection, including sample collection, metabolite extraction, an overview of liquid chromatography-tandem mass spectrometry data acquisition, 3D model generation, and finally, data visualization.

• Chemistry

  
  Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells Shawna J. Standke¹, Devon H. Colby¹, Ryan C. Bensen¹, Anthony W. G. Burgett¹, Zhibo Yang^{1 1}Department of Chemistry and Biochemistry, University of Oklahoma An integrated cell manipulation platform is developed for use in conjunction with a single-probe mass spectrometry setup for the on-line analysis of individual suspension cells under ambient conditions.

• Bioengineering

  
  Biaxial Mechanical Characterizations of Atrioventricular Heart Valves Colton Ross*¹, Devin Laurence*¹, Yi Wu¹, Chung-Hao Lee^{1,2 1}Biomechanics and Biomaterials Design Laboratory (BBDL), School of Aerospace and Mechanical Engineering, The University of Oklahoma, ²Institute for Biomedical Engineering, Science and Technology (IBEST), The University of Oklahoma This protocol involves characterizations of atrioventricular valve leaflets with force-controlled, displacement-controlled, and stress-relaxation biaxial mechanical testing procedures. Results acquired with this protocol can be used for constitutive model development to simulate the mechanical behavior of functioning valves under a finite element simulation framework.

• Engineering

  
  Magnet Assisted Composite Manufacturing: A Flexible New Technique for Achieving High Consolidation Pressure in Vacuum Bag/Lay-Up Processes Maya Pishvar*¹, Mehrad Amirkhosravi*¹, M. Cengiz Altan^{1 1}School of Aerospace and Mechanical Engineering, University of Oklahoma A new technique for applying consolidation pressure on the vacuum bag lay-up to fabricate composite laminates is described. The goal of this protocol is to develop a simple, cost-effective technique to improve the quality of laminates fabricated by the wet lay-up vacuum bag method.

• Bioengineering

  
  A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering Christopher A. Neal¹, Jennifer G. Nelson-Brantley¹, Michael S. Detamore², Hinrich Staecker¹, Adam J. Mellott^{3 1}Department of Otolaryngology, University of Kansas Medical Center, ²Stephenson School of Biomedical Engineering, University of Oklahoma, ³Department of Plastic Surgery, University of Kansas Medical Center The goal of this protocol is to demonstrate an effective method to decellularize and decalcify mouse cochleae for utilization as scaffolds for tissue engineering applications.

• Biology

  
  Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions Wei Rao¹, Ning Pan¹, Zhibo Yang^{1 1}Department of Chemistry and Biochemistry, University of Oklahoma Here, we present protocols to perform both ambient mass spectrometry imaging (MSI) of tissues and in-situ live single cell MS (SCMS) analysis using the single-probe, which is a miniaturized multifunctional device for MS analysis.

• Neuroscience

  
  Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS Taylor R. Fore¹, Audrey A. Ojwang¹, Margaret L. Warner¹, Xinyun Peng¹, Rudolf A. Bohm^1,2, William P. Welch¹, Lindsey K. Goodnight¹, Hong Bao¹, Bing Zhang^{1 1}Department of Zoology, University of Oklahoma - Norman, ²Department of Biology, Brandeis University We describe a Flippase-induced intersectional Gal80/Gal4 repression (FINGR) method, allowing tissue-specific FLP to determine Gal80 expression patterns. Wherever Gal4 and FLP overlap, Gal4 expression is turned on (Gal80 flipped out) or off (Gal80 flipped in). The FINGR method is versatile for clonal analysis and neural circuit mapping.

• Neuroscience

  
  Electrophysiology of Scorpion Peg Sensilla Elizabeth D. Knowlton¹, Douglas D. Gaffin^{1 1}Department of Zoology, University of Oklahoma This article describes an electrophysiological method for isolating chemical stimulation to individual sensilla via extracellular, tip-recordings under mineral oil.