Samuel Roberts Noble Foundation 1 article published in JoVE Immunology and Infection VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance Muthappa Senthil-Kumar1, Hee-Kyung Lee1, Kirankumar S. Mysore1 1Plant Biology Division, The Samuel Roberts Noble Foundation Virus-induced gene silencing is an useful tool for identifying genes involved in nonhost resistance of plants. We demonstrate the use of bacterial pathogens expressing GFPuv in identifying gene silenced plants susceptible to nonhost pathogens. This approach is easy, fast and facilitates large scale screening and similar protocol can be applied to studying various other plant-microbe interactions.
