3 articles published in JoVE
Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach John E. Chojnowski1, Emily A. McMillan1, Todd I. Strochlic1 1Department of Biochemistry and Molecular Biology, Drexel University College of Medicine The objective of this protocol is to label, enrich, and identify substrates of protein kinase CK2 from a complex biological sample such as a cell lysate or tissue homogenate. This method leverages unique aspects of CK2 biology for this purpose.
Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes Ferdusy Dia1, Tierra Strange1, Jenny Liang1, Jacob Hamilton1, Karen M. Berkowitz1,2 1Department of Biochemistry & Molecular Biology, Drexel University College of Medicine, 2Department of Obstetrics & Gynecology, Drexel University College of Medicine Meiosis is the developmental process by which gametes are formed through a single round of DNA replication and two successive rounds of chromosome segregation. Mammalian meiosis can be examined by utilizing a technique to prepare meiotic chromosome spreads. Here, we demonstrate a method of preparing surface-spread nuclei from mouse spermatocytes.
A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts Shaoping Hou1, Armin Blesch2, Paul Lu3,4 1Spinal Cord Research Center, Department of Neurobiology & Anatomy, Drexel University College of Medicine, 2Spinal Cord Injury Center, Heidelberg University Hospital, 3Veterans Administration Medical Center, San Diego, CA, 4Department of Neurosciences, University of California, San Diego We present a protocol for using a radio-telemetric system to record cardiovascular parameters in T4 spinal cord transected rats eight weeks after embryonic brainstem neural stem cell grafting into the lesion site. Telemetry is an advanced technique to accurately evaluate cardiovascular function in conscious freely moving spinal cord injured rats.