Allegheny-Singer Research Institute 2 articles published in JoVE Medicine Inguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets Yuanzheng Peng*1, Zhicheng Zou*1, Jiao Chen1, Hancheng Zhang1, Ying Lu1, Rito Bittino2, Hongxing Fu3, David K. C. Cooper4, Shan Lin1, Mengtao Cao1, Yifan Dai5, Zhiming Cai1, Lisha Mou1 1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Health Science Center, 2Institute for Cellular Therapeutics, Allegheny-Singer Research Institute, 3School of Pharmaceutical Sciences, Wenzhou Medical University, 4Xenotransplantation Program/Department of Surgery, University of Alabama at Birmingham, 5Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University In this protocol, a method of murine islet isolation and transplantation into the inguinal subcutaneous white adipose tissue is described. Isolated syngeneic murine islets are transplanted into a murine recipient using a basement membrane hydrogel. The blood glucose level of the recipients is monitored, and histology analysis of the islet grafts is performed. Biology A Method for Islet Transplantation to the Omentum in Mouse Ying Lu*1, Shangyou Zou*1, Suzanne Bertera*2, Rita Bottino2, David K.C. Cooper3, Zhengzhao Liu1, Yi Huang1, Chengjun Wang1, Chungu Hong1, Tian He1, Hancheng Zhang1, Qin Huo4, Hongxing Fu5, Zhiming Cai1, Lisha Mou1 1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, Shenzhen University Health Science Center, 2Institute for Cellular Therapeutics, Allegheny-Singer Research Institute, 3Xenotransplantation Program/Department of Surgery, The University of Alabama at Birmingham, 4College of Life Science and Oceanography, Shenzhen University, 5School of Pharmcy, Wenzhou Medical College A method for the omental transplantation of islets in a mouse is introduced. The isolated islets are mixed with hydrogel and the mixture is placed into the omental pouch of a diabetic mouse. Then, the blood glucose is monitored, and immuno-histochemical analysis is performed.
