2 articles published in JoVE
Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions Yancy Ferrer-Acosta*1, Maxine N. Gonzalez-Vega*1, David E. Rivera-Aponte2, Solianne M. Martinez-Jimenez1, Antonio H. Martins3 1Department of Neuroscience, School of Medicine, Universidad Central del Caribe, 2Department of Biochemistry, School of Medicine, Universidad Central del Caribe, 3Department of Pharmacology and Toxicology, Medical Sciences, Campus, University of Puerto Rico Ischemic stroke is a complex event in which the specific contribution of astrocytes to the affected brain region exposed to oxygen glucose deprivation (OGD) is difficult to study. This article introduces a methodology to obtain isolated astrocytes and study their reactivity and proliferation under OGD conditions.
A Method to Inflict Closed Head Traumatic Brain Injury in Drosophila Rebeccah J. Katzenberger1, Carin A. Loewen2, R. Tayler Bockstruck1, Mikal A. Woods3, Barry Ganetzky2, David A. Wassarman1 1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, 2Department of Genetics, University of Wisconsin-Madison, 3Department of Natural Sciences, University of Puerto Rico-Aguadilla Here we describe a method to inflict closed head traumatic brain injury (TBI) in Drosophila. This method provides a gateway to investigate the cellular and molecular mechanisms that underlie TBI pathologies using the vast array of experimental tools and techniques available for flies.