Rhode Island and Miriam Hospitals 2 articles published in JoVE Immunology and Infection SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells Collins Cheruiyot1, Zemplen Pataki1, Robert Williams1, Bharat Ramratnam2,3, Ming Li3 1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories, Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine, Warren Alpert Medical School, Brown University Here, we describe a quantitative proteomics method using the technique of stable isotope labeling by amino acids in cell culture (SILAC) to analyze the effects of HIV-1 infection on host exosomal proteomes. This protocol can be easily adapted to cells under different stress or infection conditions. Biology A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes Regina Salvat1, Leonard Moise2, Chris Bailey-Kellogg3, Karl E. Griswold1 1Thayer School of Engineering, Dartmouth College, 2Institute for Immunology and Informatics, University of Rhode Island, 3Department of Computer Science, Dartmouth College Biochemical assays with recombinant human MHC II molecules can provide rapid, quantitative insights into immunogenic epitope identification, deletion, or design. Here, a peptide-MHC II binding assay scaled to 384-well plates is described. This cost effective format should prove useful in the fields of protein deimmunization and vaccine design and development.
