3 articles published in JoVE
Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains Kathryn E. Neville1, Timothy L. Bosse1, Mia Klekos1, John F. Mills1, Marla Tipping1 1Department of Biology, Providence College We present a protocol for measuring oxygen consumption and extracellular acidification in Drosophila melanogaster larval and adult brains. A metabolic analyzer is utilized with an adapted and optimized protocol. Micro-tissue restraints are a critical component of this protocol and were designed and created specifically for their use in this analysis.
Testing for Metacognitive Responding Using an Odor-based Delayed Match-to-Sample Test in Rats Keith A. Lee1, Aidan J. Preston1, Taylor B. Wise1, Victoria L. Templer1 1Department of Psychology, Providence College This protocol describes a method for investigating the possibility of metamemory, or memory awareness, in rodents. The odor-based delayed-matching-to-sample paradigm is a novel, ecologically-relevant behavioral test useful for determining the extent to which rodents can adaptively respond based on cognitively monitoring the strength of their memory states.
Quantitatively Measuring In situ Flows using a Self-Contained Underwater Velocimetry Apparatus (SCUVA) Kakani Katija1, Sean P. Colin2,3, John H. Costello3,4, John O. Dabiri5 1Applied Ocean Physics and Engineering, Woods Hole Oceanographic Institution, 2Environmental Science and Marine Biology, Roger Williams University, 3Marine Biology Laboratory, Whitman Center, 4Department of Biology, Providence College, 5Departments of Aeronautics and Bioengineering, California Institute of Technology This protocol provides instructions on how to use a self-contained underwater velocimetry apparatus (SCUVA), which is designed for quantification of in situ animal-generated flows. In addition, this protocol addresses challenges posed by field conditions, and includes operator motion, predicting position of animals, and orientation of SCUVA.