2 articles published in JoVE
Confirmation of Myocardial Ischemia and Reperfusion Injury in Mice Using Surface Pad Electrocardiography Stephanie L. C. Scofield1, Krishna Singh1,2 1Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University, 2James H. Quillen Veterans Affairs Medical Center During murine myocardial ischemia/reperfusion surgery, correct placement of the occluding ligature is typically confirmed by visible observation of myocardial pallor. Herein, a method of electrocardiographically confirming ischemia and reperfusion, to supplement observed myocardial pallor, is demonstrated in male C57Bl/6 mice.
Using Caco-2 Cells to Study Lipid Transport by the Intestine Andromeda M. Nauli1, Judy D. Whittimore2 1Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, 2Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University Caco-2 cells can serve as an in vitro model to study the enterocyte transport of lipids, and lipid-soluble drugs/vitamins. The permeable membrane system separates the apical from the basolateral compartment, while the lentivirus expression system offers an effective gene overexpression method. The isolation of lipoproteins is confirmed by TEM.