East Tennessee State University

2 articles published in JoVE

• Medicine

  
  Confirmation of Myocardial Ischemia and Reperfusion Injury in Mice Using Surface Pad Electrocardiography Stephanie L. C. Scofield¹, Krishna Singh^{1,2 1}Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University, ²James H. Quillen Veterans Affairs Medical Center During murine myocardial ischemia/reperfusion surgery, correct placement of the occluding ligature is typically confirmed by visible observation of myocardial pallor. Herein, a method of electrocardiographically confirming ischemia and reperfusion, to supplement observed myocardial pallor, is demonstrated in male C57Bl/6 mice.

• Biology

  
  Using Caco-2 Cells to Study Lipid Transport by the Intestine Andromeda M. Nauli¹, Judy D. Whittimore^{2 1}Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, ²Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University Caco-2 cells can serve as an in vitro model to study the enterocyte transport of lipids, and lipid-soluble drugs/vitamins. The permeable membrane system separates the apical from the basolateral compartment, while the lentivirus expression system offers an effective gene overexpression method. The isolation of lipoproteins is confirmed by TEM.